Prerequisites:

BIOC 100A; Biol 110 strongly recommended.

BMB100B is part two of a three-quarter sequence, and covers topics involving enzymes, membranes and lipids. It is essentially two mini-courses; the first half will cover various aspects of enzymes, including enzyme kinetics, inhibition, allosterism, mechanisms and catalytic antibodies and RNA. The second half will deal with membrane composition and structure, specialized membrane functions, active transport and electrochemical storage, excitable membranes and neurotransmitters, membrane receptors and sensory transduction mechanisms. The first half will be taught by Tony Fink, the second by Roberto Bogomolni. The handout outlines the schedule we hope to follow.

Please be sure to have read the assigned reading in Matthews and Van Holde prior to the lecture.

Most weeks there will be a problem set due the following week. Solutions will be posted in the Science Library and on the Web. Late submissions will not be accepted. The homework problems will contribute 20% to the final grade.

There will be a midterm covering the first half of the course, and a final exam covering the second half. Each exam will count 40% toward the final grade. There will be no make-up exams except for medical reasons.

Tony Fink:

Office: 101 Sins. Labs

Phone: 9-2744

Office Hours: tba

Email: enzyme@cats.ucsc.edu or fink@chemistry.ucsc.edu

Roberto Bogomolni:

Office: 151 Sins. Labs

Phone: 9-4294

Office Hours: tba

Email: bogo@chemistry.ucsc.edu

TBA

"Biochemistry"2 nd Ed. by Mathews and Van Holde

http://www.chemistry.ucsc.edu/teaching/Winter97/ BMB100B.html

A number of books will be placed on reserve in the Science Library. These are meant as supplemental material to give greater, and more in depth, coverage then there will be time for in class, and to give you additional perspective on the subject.

We encourage you to use electronic mail for communications. We have a web page for the course, and will use it for a variety of purposes. Therefore it will be really helpful for you if you have access to the web (e. g. from any Campus instructional computer lab, or via modem from your home computer). The URL is http://www.chemistry.ucsc.edu/teaching/Winter97/BMB100B.html

WEEK OF:	SUBJECT	READING ASSIGNMENT (pages in M & VH)
January 7	Course overview, protein purification; Introduction to enzymes - overview	152-155
12	Review of chemical kinetics, Enzyme kinetics, Enzyme inhibition	360-388
19	HOLIDAY
21	Transition state theory, penicillin, ribozymes, catalytic antibodies	396-398
26	Mechanisms: Lysozyme, Serine proteases, Aspartic proteases
February 2	Coenzymes, Metalloenzymes: alcohol dehydrogenase	388-392
9	Enzyme activation: zymogens, blood clotting, Enzyme regulation: allosterism	398-408
16	HOLIDAY
17	EXCHANGE DAY MIDTERM EXAM (Chaps. ??) Review of cell structure, Biological membranes
23	Membrane structure, membrane proteins
March 2	Membrane transport, Carriers, channels, pores, Active transport, ion pumps
9	Information transfer: Hormones, Excitable membranes, neurotransmitters
16	Vision, hormones and receptors, Cellular communication: signal transduction
	FINAL EXAM

Revised 7/12/04.